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Transfection was carried out using Lipofectamine 3000 Kit on cells at 50% confluence for 6 hours, followed by protein extraction 48 hours post-transfection

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227 In another randomized placebo-controlled trial, patients with high cholesterol took 80 mg of atorvastatin (Lipitor) (a common lipid-lowering medication that blocks cholesterol production) for four weeks and then added either 180 mg of bempedoic acid or placebo to their treatment regimen for four additional weeks

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