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Every step in the mixing process introduces potential contamination if not handled correctly

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Abbreviations ASCA anti- Saccharomyces cerevisiae antibody FGID functional gastrointestinal disorders GF germ-free IEB intestinal epithelial barrier IBD inflammatory bowel disease IBS irritable bowel syndrome IBS-C constipation-predominant irritable bowel syndrome IBS-D diarrhea-predominant irritable bowel syndrome IBS-M irritable bowel syndrome with mixed bowel habit LPS lipopolysaccharides SCFA short-chain fatty acids