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The free Ca 2+ concentrations of the lysis and stimulation buffer were estimated at 121 nmol/l (for 1.65 mmol/l CaCl 2 ) and 1.2 to 1.4 mol/l (for 6.65 mmol/l CaCl 2 ), respectively, using BAD4-software [25]

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Kennedy has instead focused on lifestyle changes for combating obesity, though he told CNBC last year GLP-1s "have a place" in treating obesity

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