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Later in 2014, with the support of Food and Drug Administration (FDA) and European Medicines Agency (EMA), the PSTC included neutrophil gelatinase-associated lipocalin (NGAL) and osteopontin (OPN) for further evaluation as emerging biomarkers (Dieterle et al

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RNA extraction, RT-PCR, and real-time PCR Total RNA extraction was performed using the Maxime RT Premix Kit (iNtRON Biotechnology) according to the manufacturers instructions

Crdoba-Aguilar A, Ruiz-Silva D, Mungua-Steyer R, Lanz-Mendoza H (2011) Do reproductive activities compromise immunological competence as measured by phenoloxidase activity

This is the approach we took with GLP-1 Up