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For the OGD phase, cells were washed twice with PBS and then incubated in glucose-free DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) under hypoxic conditions (1% O 2 , 94% N 2 , 5% CO 2 ) in a hypoxia chamber (BioSpherix, Parish, NY, United States) for 8 h

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Accessed 20 Jan 2020

JAMA 311 , 16701683

It exhibits resistance to enzymatic degradation in certain experimental conditions, which has made it an attractive compound for studies involving peptide stability and systemic biological activity

Parseghian CM, Loree JM, Morris VK, Pereira AAL, Vilar Sanchez E, Kee BK, Raghav KPS, Dasari A, Wu J, Raymond VM, Banks K, Talasaz AA, Lanman RB, Overman MJ, Kopetz S