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Protect from High Temperatures Exposure to temperatures above 46F (8C) can lead to a faster breakdown of the medication, reducing its potency

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10.1097/CM9.0000000000001712 Chin Med J

After three washes with PBS, the sections were pre-incubated in blocking solution (5% serum albumin, 2% normal goat serum, and 0.3% Triton X-100 in PBS) for 20 min at room temperature, then incubated with an anti-c-Fos rabbit antibody (#sc-52, 1:2000

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