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LOD was determined at 75C using a Mettler LP 16 infrared dryer (Hightstown, NJ) equipped with a Mettler PM 400 balance and a Mettler GA45 printer

Additionally, ascorbic acid has other well-established biological roles, including serving as a cofactor for several essential enzymes, such as hydroxylases (Levine et al

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New England Journal of Medicine, 368(2), 149-160

The labile iron pool is present in mitochondria, lysosomes, cytosol and the nucleus and can be regulated by the absorption, distribution and export of iron in the cell ( 2+ to Fe 3+ (Figure 2)