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The quantitative assessment of RNAi effector molecules, including siRNA and shRNA, is crucial for the progression of RNAi-based treatments, as it evaluates delivery efficiency and pharmacokinetics, hence serving as a vital element in both preclinical and clinical research

Inaccurate dosing leads to skewed, unreliable data

Subsequent heightened reflex responsiveness may occur

Experimental work in isolated vascular tissue and cell culture models indicates interactions involving regulators such as endothelial nitric oxide synthase, Src kinase signaling, and vascular endothelial growth factor receptor pathways

2014;24(10):R45362

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