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Culture media from 24-hour incubated cells were collected, stored at -80 C, and analyzed for urea concentration using LC/MS/MS API3000, normalized to total cell protein

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That being said, Dihexa was administered to the rats in doses of 1.44 mg/kg of 2.88 mg/kg in order to see how levels of AngIV changed in response to the nootropic compound

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Dihexa is a research peptide commonly studied in laboratory environments involving peptide structure analysis, neurological compound research, and biochemical studies