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If resistance or signs of distress are met during gavage insertion, withdraw the gavage and start the procedure again if appropriate to do so.[4] The mouse should be euthanised upon fluid entering the lungs or rupture to the pharynx or oesophagus.[1, 4] Figure 5

Use what remains in the old vial at the old concentration, then start the new vial fresh

Folic Acid Folate (vitamin B9) is a cofactor in amino acid metabolism reactions (aka methylation reactions) and helps regulate DNA synthesis and gene expression