The regulatory effect of GPX8 on Ca 2+ may be related to the inositol 1,4,5-triphosphate receptor (IP3R) effect, and the transmembrane domain (TMD) of GPX8 is thought to play a key role in the regulation of Ca 2+ signaling (Yoboue et al., 2017)
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Immunohistochemistry Five tissue microarrays slides of the 122 cases of malignant peripheral nerve sheath tumor, WTS slides of the 40 cases of malignant peripheral nerve sheath tumor (matched with those in tissue microarrays of malignant peripheral nerve sheath tumor), WTS slides of the 28 cases of primary desmoplastic/spindle cell melanomas, tissue microarrays slides of the 8 cases of primary melanomas and tissue microarrays slides of 229 cases of metastatic melanomas were deparaffinized in xylene, hydrated in alcohol, and baked in a microwave (30 min in Tris-buffer, pH 9)