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The cDNA for SLC7A11 coding region was amplified from ZR-75-1 breast cancer cell line using standard RT-PCR and ligated into EcoR I- Sal I linearized retroviral pBabe-hygro vector, generating the pBabe-hygro-SLC7A11

4 As the numbers on the scale decrease, however, a common and valid question arises: "Am I losing muscle along with the fat?" It's important to understand that losing some muscle, or more accurately, lean body mass (which includes muscle, water, bone, and organs), is a typical aspect of weight loss, regardless of the method used

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