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In an aspect of second embodiment, the said insoluble tag is optionally linked to a nucleotide sequence encoding affinity tag to give Formula (III), SUBSTITUTE SHEETS (RULE 26) Affinity Tag Insoluble tag Fusion tag Semapeptide (Nucleotide Sequence) Formula (III) In the third embodiment, the present invention is related to increase in accumulation of resulting semapeptide by fermentation process