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body condition score 2.53.5) were used for the fertility trial

On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

More serious complications are rare but could include infiltration (IV fluid leaking into surrounding tissue), phlebitis (inflammation of the vein), infection (extremely rare with proper technique), or allergic reactions to ingredients