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Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)

are currently in shortage as of 2024, leaving patients with delays or a complete lack of access to these medicines

Parasitol Int 57 , 17, doi: S1383-5769(07)00101-8 (2008)

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Some probiotics also produce butyrate a short-chain fatty acid important for health of cells within the colon wall (see below) (Sartor 2011)