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Briefly, the Nb35 target gene was transformed in BL21 and grown in a TB culture medium with 100 g/mL ampicillin, 2 mM MgCl 2 , and 0.1% (w/v) glucose at 37 C, 180 rpm

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2025 Nov 11:17:100228 doi: 10.1016/j.obpill.2025.100228

One of the two letters sent to 503A pharmacies also includes this recommendation