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Both groups were on the same calorie-restricted diet

doi: 10.1111/jocd.14983

Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 l volume containing 7.5 l eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 l 5 RT buffer (Promega), 30 U RNasin RNase inhibitor (Promega), and 0.75 g random hexadeoxynucleotides (Roche) in order to retrotranscribe the whole HCV genome preventing any bias during the reaction

In ASD children, this reaction is impaired by a reduction of vitamin B12 levels probably attributed to a reduced oral intake, gut dysbiosis or autoimmunity

"It was frightening for my family to see me like that," she said