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In order to ascertain the optimal time point, lapatinib at the concentration of 10 M was treated in the different time points: pretreatment for 6 h (pre 6 h), pretreatment for 2 h (pre 2 h), co-treatment with Glu or erastin (pre 0 h) and posttreatment for 2 h (post 2 h)

The folding paradigm stipulated that unfolded molecules will always spontaneously fold into the same shape

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Thus, the amount of LC3-II is correlated with the autophagy level and is a reliable marker for monitoring autophagy-related processes 21