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Activated microglia release chemokines, cytokines, and reactive oxygen species, crucially contributing to neuroinflammatory cascades while disrupting neurotransmitter balance and synaptic plasticity through pro-inflammatory phenotypic transformation

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Absorbance at 450 nm was then measured using a microplate reader, with lower absorbance indicating reduced viability

The comorbid conditions of Fragile X syndrome, tuberous sclerosis, phenylketonuria, or fetal alcohol syndrome were excluded because these children present some autistic behavioral features, but the origin is known

CONTRAINDICATIONS:the product is not recommended for pregnant and breastfeeding women