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After incubation at 37C, proteins in the reaction mixture were removed using a centrifugal ultrafiltration device (molecular weight cut-off, 10 kD

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doi: 10.1002/jcph.1008

In the bile ducts bacterial glucuronidases will produce unconjugated bilirubin from bilirubin glucuronide with formation of insoluble calcium bilirubinate stones when the unconjugated bilirubin complexes with calcium