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Extracellular GPX3 relies on the presence of a cysteine encoded by the UGA opal codon in its active catalytic site to undergo conversion into a functional protein (19)

Patients with pre-existing gastroparesis prior to medication initiation were also excluded

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Neurons were transfected with mRFP-P-actin on day in vitro 6 (DrV6) using LipofectAMINE 2000 (Invitrogen) according to the manufacturer's protocol