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coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

described three mechanisms that ultimately activate Th1 autoreactive lymphocytes to link Hp and HT

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doi: 10.3389/fphys.2020.00794 Received 14 May 2020 Accepted 15 June 2020 Published 09 July 2020 Volume 11 - 2020 Edited by Giuliano Ciarimboli, University of Mnster, Germany Reviewed by Katarzyna A

Whats more, cardiac lipotoxicity causes insulin dysregulation and glycometabolism impairment (Nakamura and Sadoshima, 2020), which are speculated to provide a metabolic arrhythmogenic substrate for AF (Maria et al., 2018)

2.2 Isolation of Oocytes and Preimplantation Embryos (O/PEs) The isolated O/PEs were washed in FHM medium [28], containing 1% bovine serum albumin (BSA)