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ab27325) and for detection of -actin, an HRP-conjugated anti--actin antibody (Sigma Aldrich, Louis, MO, USA

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After denaturation in Laemmli buffer (Tris 40 mM, DTT 100 mM, sodium dodecyl sulfate 2% (v/v), glycerol 5% (v/v), bromophenol blue 0.075% (m/v), pH 6.8), proteins were separated on a 4-12% Criterion XT Precast Gel (Bio-Rad) in XT MOPS buffer (Bio-Rad) at 120 V for 2 h

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