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PBMCs isolated from individuals with T2DM at baseline and three months post-supplementation with either L-GSH or empty liposomes were used for generating in vitro granulomas

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Immunoassay Tear fluid was extracted from half of the remaining strip by agitating small cut pieces of these strips in 100 L PBS, Tween20 0.05% and cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) at 4 C for 1.5 h 28

Manufacturer-supplied solutions of horseradish peroxidase, Amplex Red, and lactate oxidase were added to samples in wells of a 96-well microplate which was then incubated at room temperature for 30 min

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