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For the detection of mitochondrial membrane potential, the cells were incubated with DMEM without phenol red containing 10 g/mL JC-1 dye for 10 min at 37 C and were then washed with PBS 3 times to remove any residual dye

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This is achieved through: Dietary changes : reducing refined carbohydrates, saturated fats, and overall calorie intake Regular physical activity : at least 150 minutes of moderate-intensity exercise weekly, as recommended by UK Chief Medical Officers' guidelines Managing underlying conditions : optimising control of diabetes, hypertension, and dyslipidaemia to reduce cardiovascular risk Avoiding hepatotoxic substances : ideally avoiding alcohol entirely if you have liver disease

The formula also includes NAC and vitamin B6, two important nutrients that support the body's natural production of glutathione