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The peptide may still produce some biological activity, but the response will be weaker and more variable than expected, introducing uncontrolled variability into the research data

Results None of the samples contained any measurable semaglutide

All qRT-PCR samples were run in technical duplicates and the Ct method based on the reference value estimate from 3 housekeeping gene (GAPDH, GUSB and TBP) was used to calculate relative expression level of each gene and reaction

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