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1625 In contrast, structures of other class B1 GPCRs bound to calcitonin (CT), 26 calcitonin gene related peptide (CGRP), 27 maxadilan, 20 corticotropin-releasing factor (CRF) 22 or urocortin-1 (UCN1) 28 reveal a loop secondary structure near the agonist N-terminus, followed by either a short (CT-family peptides) or extended -helix

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Isolation of nuclear and cytoplasmic proteins Nuclear and cytoplasmic proteins were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit following the detailed instructions provided by the manufacturer (Thermo scientific, Rockford, IL, USA)

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