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There are also instances of the use of the customized medium Nycoprep 1.068 [10,11], which was designed for the purification of monocytes from a human leukocyte-rich plasma

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Nishino, H., Tokuda, H., Satomi, Y., Masuda, M., Osaka, Y., Yogosawa, S., Wada, S., Mou, X.Y., Takayasu, J., Murakoshi, M., & Jinnno, K

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The mid 20 th century recorded the first indications that the hypothalamus plays a major role in feeding behaviour and energy homeostasis, whereby the electrical stimulation of the ventromedial hypothalamus (VMH) suppresses food intake, and the bilateral lesions of these structures induce hyperphagia and obesity