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After 15 min, 0.1% DMSO or 8.4 M HC were added in each well and the fluorescence intensity of the dye was measured for additional 15 min

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The form and protocol are individualized

PMID 12818476

Injection and incubation of eggs: In this study, four doses of L-carnitine (0.0, 40, 80 and 120 mg dissolved in 1 mL of sterilized saline) were injected in fertilized eggs at three incubational ages (14, 16 and 18 days of incubation)