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Copper-peptide complex formation and molecular identity should be verified by LC-MS (liquid chromatography-mass spectrometry)

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LaccID was more stable than our starting template LacAnc100, with a T 50 (temperature at which the enzyme loses 50% of activity after 10 min of incubation) of 57.8 0.5 C compared to 54.8 0.2 C for LacAnc100 (Fig

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Immunohistochemistry revealed S-100 protein, which was diffusely and strongly positive