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After three washes with PBS, SPZs were stained for 1h at RT with a combination of 0.5 M DAPI (4,6-diamidino-2-phenylindole) and 1:400 dilution of donkey Alexa Fluor 488 anti-sheep IgG (A-11015, Invitrogen)

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Monitoring and adjusting supplementation Test b12 levels every 6 to 12 months during semaglutide use

Because the peptide occurs naturally in human plasma (at roughly 200 ng/mL in young adults, declining to approximately 80 ng/mL by age 60), topical supplementation essentially restores youthful local concentrations in treated skin