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Kopera M, Gwozdzinski K, Pieniazek A

The tar phase of cigarette smoke contains long-lived radicals, while the gas phase contains short-lived radicals such as nitric oxide and superoxide

Total RNA was extracted from with Trizol reagent (Beyotime, Shanghai, China) according to the manufacturers instructions

2.8 Scratch assay in vitro To assess the impact of a GLUT1 inhibitor on macrophage cell migration, an in vitro scratch assay was employed as a direct and cost-effective method

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