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Cells were subcultured twice a week at a ratio of 1:5 to 1:8.Transient transfection of HeLa cells (12 x 10 5 cells/6-well) with pcDNA3.1(+) NAPstar or pSC2 HyPer7 (Addgene

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Notably, ischemic reactive hyperemia (IRH), which is used to assess microvascular endothelial reactivity, improved significantly in the MeDE group, indicating that the combination of diet and exercise interventions can enhance vascular endothelial function, thereby improving atherosclerosis ( Moreover, intermittent fasting (IF), as a metabolic intervention strategy, has been extensively studied and has been shown to significantly improve body weight, lipid profiles, and blood pressure, thereby reducing the risk of CVDs (Kazeminasab et al., 2024)

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