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doi: 10.3390/cimb46080521

Once reaching near-confluence, culture medium was aspirated and cells were washed once with PBS, followed by a 20 min incubation with NucBlue Live ReadyProbes Reagent (#R37605, ThermoFisher Scientific, Waltham, MA, USA) to stain the nuclei

22,30 Even though the efficacy of radiation therapy and chemotherapy has yet to be proven, these treatment methods are recommended because of the aggressive potential of these lesions