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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

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This cascade results in the cleavage of Gasdermin D, leading to the formation of membrane pores that may contribute to cell lysis and the release of the proinflammatory cytokines interleukin (IL)-1 and IL-18

Reverse pushups, pull-ups, squats, bicep curls and 10 minutes of high-intensity interval training (HIIT) are among the exercises on the docket

doi: 10.1038/s41556-018-0178-0

& Gori Savellini, G