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After overnight incubation, the membranes were washed three times with TBS-T and then incubated for 1 h at room temperature with horseradish peroxidase (HRP)-conjugated secondary antibodies: anti-Rabbit IgG-HRP (1:5000

Potential Research Applications: pH regulation and buffer system studies Topical antimicrobial and cytotoxicity research Reagent dilution and formulation preparation Laboratory protocol requiring acidic conditions For laboratory use only

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