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While numerous studies have extensively examined the effects of iron overload on cancer initiation and progression, the potential relationship between ID and cancer has been largely overlooked and remains poorly defined

Freeze-thaw sensitivity is lower than for a peptide, since there is no secondary structure to lose, but aliquoting still avoids repeated stopper punctures and concentration drift from evaporation

PLoS ONE 14 , e0214088 (2019)

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[DOI] [PubMed] [Google Scholar] 238.Schoenen J., Lenaerts M., Bastings E

The oocytes were subsequently incubated in a permeabilizing solution (0.5% Triton-X-100 in PBS-PVA) for 30 min at room temperature, washed twice in PBS-PVA, and then blocked with 2% FCS in PBS for 45 min