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That GPCRs exhibit constitutive activity in the absence of any agonist has been proposed to explain why certain ligands, originally thought to be antagonists, may act to inhibit basal activity of unoccupied receptors (i.e., they are inverse agonists) (Costa and Cotecchia, 2005)

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Ultrastructural analysis Graft samples were fixed in glutaraldehyde, dehydrated, impregnated, embedded in epoxy resin and acrylic, and cut into 60-nm sections using an ultramicrotome