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Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) was performed using a QuantiTect SYBR-Green RT-PCR kit (Qiagen, Venlo, The Netherlands) and a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA)

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Having better control on body weight and diabetes are the key reasons for cardiovascular health improvement on GLP-1 medications