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The strokes were categorized according to the Trial of ORG 10172 in Acute Stroke Treatment (TOAST) [24]

Hypertension, ischemic heart disease, patients with atherosclerosis and coronary artery disease, diabetes, chronic lung diseases, smoking and obesity are associated with low baseline GSH levels and reduced GSH/oxidized glutathione (GSSG) ratios (Khanfar and Al Qaroot, 2020

It also functions as an antioxidant by delivering non-toxic bioavailable copper to cells while preventing free radical activity from unbound ionic copper

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ab178860, Abcam) overnight at 4 C followed by incubation with a horseradish peroxidase-linked secondary antibody

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