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Baseline testing before beginning any protocol establishes reference points for comparison

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Occasional small leakage isnt concerning, but a pattern suggests a technique issue worth discussing

Consistent with previous observations 13 , we demonstrated that exendin-P5 exhibited faster G s conformational transitions, relative to GLP-1 and exendin-4, and this was coupled with faster dissociation of the G protein heterotrimer in the Nanobit assay (Figure 7B-C)