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Protease activity was quenched by acidification with trifluoroacetic acid (TFA) to a final concentration of approximately 1% and the resulting peptide mixture was concentrated using reversed-phase Sep-Pak C18 cartridge (Waters)
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Nevertheless, given the strong binding activity of the fragment derivative 27 in the CPMG assay, a pure stock of 27 was synthesised and put forward for further characterisation using a quantitative LO-NMR assay to determine the dissociation constant
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Temperature excursions above 8C denature the peptide structure irreversiblya concern for athletes traveling or storing peptides in gym bags
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