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Conversely, when fluid intake is inadequate or fluid losses are excessive, the kidneys conserve water by producing more concentrated urine, which appears darker yellow or amber

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Therefore, as an alternative to URA3 (above), a strain (designated pGSH1-GAP1 in the following context) was constructed for using GAP1 as the counter-selectable marker to enrich phenotypically high-GSH producers, via replacement of the native pGAP1 promoter with pGSH1 (Fig