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Endocrine 66 (2), 157165 (2019)

As soon as the supernatant was centrifuged, 400 mL of the supernatant was poured into the corresponding EP tube and stored overnight at 20 C in the refrigerator before being centrifuged at 12,000 r/min for 3 min at 4 C, and approximately 2000 mL of supernatant was collected in the injection bottle liner for on-board analysis

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