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Analysis of neutrophil gelatinase-associated lipocalin (NGAL) in mouse urine Concentrations of NGAL/Lcn-2 were determined in urine samples using the mouse lipocalin-2/NGAL DuoSet ELISA kit plus the DuoSet ELISA Ancillary Reagent Kit 2 from R&D systems (DY1857 and DY008), according to manufacturers protocol

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- Reconstituted: add bacteriostatic water (0.9% benzyl alcohol) slowly down the inner wall of the vial

11e, f), likely due to a ceiling effect from maximal 2MT-induced analgesia

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