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In experiments aiming to capture more interacting proteins, the beads were washed five times with 0.5 ml of cold lysis buffer (50 mM Tris-HCl, 0.8% Igepal-CA630, 5% glycerol, 150 mM NaCl, 1.5 mM MgCl2, pH 7.5, 1 mM DTT, cOmplete mini protease EDTA-free inhibitor) containing additional 0.2% NP-40, referred to as soft wash in this manuscript

During this period, most patients report stable hunger signals, improved satiety between meals, and meaningful weight reduction each month

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Our LC patients were evaluated based on diagnostic criteria developed by CDC and WHO (30, 31) for ME/CFS