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Louis, United States), Precision Count BeadsTM (BioLegend, San Diego, United States), Fixation/Permeabilization Concentrate (Invitrogen, Carlsbad, United States), PercollTM (GE Healthcare, Little Chalfont, Buckinghamshire, UK), ACK lysing buffer (Gibco, Grand Island, United States), Triton X-100 (Sangon Biotech), Tissue-Tek O.C.T

Mechanistic Comparison# Dosing Comparison# Amycretin Dosing# Amycretin dosing is investigational: Subcutaneous: Once-weekly injection, doses up to 60 mg studied in phase 1b/2a over 36 weeks Oral: Daily tablet, doses up to 50 mg (or 2 x 50 mg) studied in phase 1 Phase 2 T2D: Both subcutaneous and oral formulations studied over 36 weeks Phase 3 dose selection will be informed by the completed phase 2 data

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