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Acknowledgements This work was supported by the Intramural Research Program of the NIH/National Institute of Environmental Health Sciences 1Z01ES090079

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Enzymatic Reaction Conditions The substrate solution used for the NsPCS reaction was prepared as follows: 28.7 mg of tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Tokyo Chemical Industry) and 3.0733 g of GSH (Nacalai Tesque) were weighed in a 100 mL volumetric flask, 20 mL of 1000 mM potassium phosphate buffer (pH 8.0) was added, and the solution was gently shaken

J Biol Chem (2003) 278(19):168028

cysteine and