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Since AAVs have been found to integrate within DSBs with high frequency [152], the coupling of CRISPR-Cas9 with AAVs as the delivery vector must be carefully evaluated before clinical translation [58, 153]
doi: 10.3390/cells10061376 18 HartmanMLFariaACVanceMLJohnsonMLThornerMOVeldhuisJD
Materials and Methods Animals and Drugs Eight-week-old male BKS-Lepr em2Cd479 /Gpt (db/db) mice and C57BL/6N mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China)
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