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Consider oxidative stress markers (8-OHdG, MDA), direct glutathione measurement (GSH:GSSG ratio), mitochondrial function indicators, inflammatory markers (hs-CRP, IL-6), and genetic assessment of detoxification capacity

The darker the roast, the stronger and more bitter the taste of the coffee

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The immunoblots were incubated with primary antibody at 4 C overnight followed by incubation with IR Dye-coupled secondary antibody and visualization using the LI-COR Odyssey Fc imaging system

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